Journal:

Article Title: An Orphan Seven-Transmembrane Domain Receptor Expressed Widely in the Brain Functions as a Coreceptor for Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus

doi:

Figure Lengend Snippet: Expression of APJ in human tissues. Membranes containing poly(A)+ RNA from various human brain regions (A) and peripheral tissues (B) (obtained from Clontech) were incubated with a labeled cDNA probe specific for APJ overnight and then exposed to a Fuji Imaging plate for 4 h. (A) Lanes: 1, amygdala; 2, caudate nucleus; 3, corpus callosum; 4, hippocampus; 5, whole brain; 6, substantia nigra; 7, subthalamic nucleus; 8, thalamus; 9, cerebellum; 10, cerebral cortex; 11, medulla; 12, spinal cord; 13, occipital lobe; 14, frontal lobe; 15, temporal lobe; 16, putamen. (B) Lanes: 1, spleen; 2, thymus; 3, prostate; 4, testis; 5, ovary; 6, small intestine; 7, colonic mucosa; 8, total PBLs.

Article Snippet: Membranes containing poly(A) + RNA from various human brain regions (A) and peripheral tissues (B) (obtained from Clontech) were incubated with a labeled cDNA probe specific for APJ overnight and then exposed to a Fuji Imaging plate for 4 h. (A) Lanes: 1, amygdala; 2, caudate nucleus; 3, corpus callosum; 4, hippocampus; 5, whole brain; 6, substantia nigra; 7, subthalamic nucleus; 8, thalamus; 9, cerebellum; 10, cerebral cortex; 11, medulla; 12, spinal cord; 13, occipital lobe; 14, frontal lobe; 15, temporal lobe; 16, putamen. (B) Lanes: 1, spleen; 2, thymus; 3, prostate; 4, testis; 5, ovary; 6, small intestine; 7, colonic mucosa; 8, total PBLs. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5 caption a7 Expression of APJ in primary cells and in cell lines.

Techniques: Expressing, Incubation, Labeling, Imaging

Journal: Nucleic Acids Research

Article Title: The evolution and expression of the snaR family of small non-coding RNAs

doi: 10.1093/nar/gkq856

Figure Lengend Snippet: Differential expression of snaRs in brain and other tissues. Relative expression of snaR-A ( A ), snaR-C ( D ) and snaR-G2 ( E ) in human tissues and of snaR-A ( B ) and snaR-G2 ( F ) in regions of the human brain as determined by qPCR analysis. Histograms are averages of 3–4 separate experiments each performed in duplicate and normalized to placental sample. Standard deviations are shown. Two antipodal slices (A and B) were made for each brain tissue, ‘placenta A and B’ represent two different purchased lots. ( C ) Northern blots of total RNA extracted from 293 cells and testis, probed for snaR-B and -C. End-labeled 293 cell total RNA served as marker (M).

Article Snippet: Adrenal gland (Adr), fetal brain (Fbra), fetal kidney (Fkid) and mammary gland (Mam) human tissue total RNA was purchased from Clontech, while adipose (Adi), bladder (Bla), brain (Bra), cervix (Cer), colon (Col), esophagus (Eso), heart (Hrt), kidney (Kid), liver (Liv), lung (Lun), post-menopausal ovary (Ova), term placenta (Pla), prostate (Prs), skeletal muscle (SM), small intestine (Sint), spleen (Spl), testis (Tes), thymus (Thm), thyroid (Thr) and trachea (Tra) human tissue total RNA were purchased from Ambion.

Techniques: Expressing, Northern Blot, Labeling, Marker

Journal: Nucleic Acids Research

Article Title: The evolution and expression of the snaR family of small non-coding RNAs

doi: 10.1093/nar/gkq856

Figure Lengend Snippet: Subcellular localization of snaR-A. ( A ) Total RNA isolated from 293T, 293 and HeLa S3 cell nuclear and cytoplasmic extracts was examined for snaR-A by northern blotting (top panel). Extracts were immunoblotted for PARP (middle panel) and α-tubulin (bottom panel). Asterisk denotes a possible snaR-A conformer band. ( B ) Northern blot of 293 cell and testis total RNA, probed for snaR-A. ( C ) Co-sedimentation of snaR-A with ribosomes. HeLa cell extract was fractionated in a sucrose gradient and analyzed by northern blotting for snaR-A (top panel) and 5S rRNA (middle panel), and by immunoblotting for NF90 (bottom panel). The percentage of snaR-A in three regions of the gradient was determined by phosphorimager quantitation of the blot. Blot is duplicated from with permission. ( D ) Extract of HeLa cells incubated with puromycin was analyzed as in C .

Article Snippet: Adrenal gland (Adr), fetal brain (Fbra), fetal kidney (Fkid) and mammary gland (Mam) human tissue total RNA was purchased from Clontech, while adipose (Adi), bladder (Bla), brain (Bra), cervix (Cer), colon (Col), esophagus (Eso), heart (Hrt), kidney (Kid), liver (Liv), lung (Lun), post-menopausal ovary (Ova), term placenta (Pla), prostate (Prs), skeletal muscle (SM), small intestine (Sint), spleen (Spl), testis (Tes), thymus (Thm), thyroid (Thr) and trachea (Tra) human tissue total RNA were purchased from Ambion.

Techniques: Isolation, Northern Blot, Sedimentation, Western Blot, Quantitation Assay, Incubation

Journal: Nucleic Acids Research

Article Title: The evolution and expression of the snaR family of small non-coding RNAs

doi: 10.1093/nar/gkq856

Figure Lengend Snippet: snaR-A induction by cell transformation and immortalization. Derivation of pre-immortal and immortal SV40-transformed cell lines from HS74 and HSF43 normal diploid fibroblasts is shown schematically, as described in the text and Methods. Senescent HS74 cultures (HS74 sen) are also represented. Northern blots of total RNA were probed for snaR-A (top), BC200 (middle) and 5S rRNA (bottom). Human testis (Tes) and fetal brain RNA (Fbra) were included as controls. Intensities of snaR-A and BC200 bands were determined relative to parental cells by phosphorimager quantification and the ratio of snaR-A to BC200 was calculated.

Article Snippet: Adrenal gland (Adr), fetal brain (Fbra), fetal kidney (Fkid) and mammary gland (Mam) human tissue total RNA was purchased from Clontech, while adipose (Adi), bladder (Bla), brain (Bra), cervix (Cer), colon (Col), esophagus (Eso), heart (Hrt), kidney (Kid), liver (Liv), lung (Lun), post-menopausal ovary (Ova), term placenta (Pla), prostate (Prs), skeletal muscle (SM), small intestine (Sint), spleen (Spl), testis (Tes), thymus (Thm), thyroid (Thr) and trachea (Tra) human tissue total RNA were purchased from Ambion.

Techniques: Transformation Assay, Northern Blot